1. A nicked RNA molecule can be ligated by
A. T4 RNA ligase
B. DNA polymerase
C. T4 DNA ligase
D. all of these
Answer: C
2. The tertiary structure of yeast tRNA
A. involves extensive base stacking interactions
B. resembles the 3-dimensional structure of other tRNAs
C. is maintained mostly by non-Watson-Crick base pairing
D. all of the above
Answer: D
3. Which of following RNA characteristically contains unusual purines and pyrimidmes?
A. rRNA
B. nRNA
C. mRNA
D. tRNA
Answer: D
4. The anticodon is a structure on
A. mRNA.
B. tRNA.
C. aribosome.
D. rRNA.
Answer: B
5. The genetic material of retroviruses such as HIV is
A. DNA
B. RNA
C. protein
D. all of these
Answer: B
6. Retroviruses replicate via __________ intermediate
A. RNA
B. DNA
C. mRNA
D. rDNA
Answer: B
7. During RNA synthesis, the DNA template sequence 5’Tp Ap Gp Cp 3' Would be transcribed to produce which of the following RNA sequence?
A. 5'-Ap Tp Cp Gp-3'
B. 5'-Gp Cp Up Ap-3'
C. 5'-Gp Cp Tp Ap-3'
D. 5'-Ap Up Cp Gp-3'
Answer: B
8. RNA instability in alkaline solutions is due to
A. adenine
B. ribose
C. uracil
D. single strand nature
Answer: B
9. In RNA, uracil pairs with
A. adenine
B. cytosine
C. thymine
D. guanine
Answer: A
10. Two features of the tRNA molecule associated, in converting the triplet codon to an amino acid, are
A. in the T Loop and D stem and loop
B. in the anticodon loop and D stem loop
C. in the anticodon loop and the 3' CCA end
D. none of the above
Answer: C
11. Which of the following is the smallest of the RNAs?
A. Messenger RNA
B. Transfer RNAs
C. Ribosomal RNAs
D. All of these
Answer: B
12. The mRNA from which of the following would contain a poly-A tail?
A. A restriction endonuclease from E. coli
B. Bacterial alpha-amylase
C. Human insulin
D. Bacteriophage DNA ligase
Answer: C
13. What is the average size (in bp) of a mature t-RNA?
A. 80 bp
B. 100 bp
C. 120 bp
D. 140 bp
Answer: A
14. What modified base is at the 5' extremity of a capped eukaryotic m-RNA?
A. 1-methyl-adenosine
B. 2'-O-methyl-guanosine
C. 7-methyl-guanosine
D. 1-methyl-guanosine
Answer: C
15. What is the function of messenger RNA?
A. It carries amino acids
B. It is a component of the ribosomes
C. It is a direct copy of a gene
D. It is the genetic material of some organisms
Answer: C
