MCQ on Genetic Engineering
1. In a chromosome , there is a specific DNA sequence , which is responsible for initiating replication , known as
( a ) vector
( b ) origin of replication
( c ) recognition sequence
( d ) blunt end
Ans. b
2. When an alien DNA is linked with the origin of replication , so that this alien piece of DNA can replicate and multiply itself in the host organism , then this can be called as
( a ) gene transfer
( b ) gel electrophoresis
( c ) cloning
( d ) transformation
Ans. c
3. It was found that Hind II always cuts DNA molecules at a particular point by recognising a specific sequence of six base pairs . This specific base sequence is known as the
( a ) recognition sequence
( b ) restriction endonuclease
( c ) palindromic nucleotide sequence
( d ) nucleases
Ans. a
4. The cutting of DNA by restriction endonucleases results in the fragments of DNA . These fragments can be separated by a technique known as
( a ) PCR
( b ) molecular diagnosis
( c ) isolation of DNA
( d ) gel electrophoresis
Ans. d
5. Now – a – days , the most commonly used matrix in gel electrophoresis is
( a ) agarose
( b ) ethidium bromide
( c ) sulphate bromide
( d ) sulphite bromide
Ans. a
6. The separated DNA fragments can be visualised only after staining the DNA with a compound known as
( a ) ethidium sulphide
( b ) ethidium bromide
( c ) ethidium sulphite
( d ) ethidiurn bromite
Ans. b
7. You can see bright orange a coloured bands of DNA in a ethidium bromide stained gel exposed to
( a ) X – rays
( b ) Sun rays
( c ) UV – rays
( d ) Y – rays
Ans. c
8. In addition to ori , the vector requires which helps in identifying and elimination non transformants and selectively permitting the growth of the transformants
( a ) solectia
( b ) cloning sites
( c ) antibiotic resinance genes
( d ) bacteriophage
Ans. a
9. Retroviruses in animals have the ability to transform normal cell into
( a ) tumour
( b ) RNA containing cell
( c ) DNA containing cell
( d ) cancerounce
Ans. d
10. The method , in which recombinant DNA is directly injected into the nucleus of an animal cell is
( a ) microinjection
( b ) blolistic
( c ) gene gun
( d ) Both ( b ) and ( c )
Ans. a
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11. Which of the following processes is employed to check the progression of a restriction enzyme digestion ?
( a ) RFLP
( b ) PCR
( c ) Agarose gel electrophoronis
( d ) Genetic printing
Ans. c
12. A thermostable DNA polymerase , which remains active during the high temperature induced denaturation of double – stranded DNA is isolated from a bacterium
( a ) E col
( b ) Thermus aquaticus
( c ) Salmonella
( d ) Thermus coccus
Ans. b
13. If any protein encoding gene is expressed in a heterologous host , is called a
( a ) recombinant protein
( b ) primary protein
( c ) secondary protein
( d ) tertiary protein
Ans. a
14. A stirred – tank reactor is usually cylindrical or with a curved base to
( a ) give a large surface area
( b ) facilitate the mixing of the reactor content
( c ) avoid the mixing of the reactor content
( d ) increase the oxygen transfer area
Ans. b
15. The processes , which are collectively referred to as downstream processing are
( a ) identification and purification
( b ) separation and puntication
( c ) selection and separation
( d ) All of the above
Ans. b
16. The process by which multiple copies of the gene of interest is synthesised in vitro using two sets of primers , is known as
( a ) PCR
( b ) gol electrophoresis
( c ) downstrearn processing
( d ) genetic printing
Ans. a
17. In order to link the alien DNA , the vector needs to have very few , preferably single ……. for the commonly used restriction enzymes
( a ) ori
( b ) recognition site
( c ) insertional sites
( d ) circular sites
Ans. b
18. E coli cloning vector pR322 contains restriction sites ( Hind III . Eco RI , Bam HI . Sal 1. Puu II . Pst L Cla 1 ) , ori , amp R . tet B and rop . Rop codes for the
( a ) antibiotic resistance gones
( b ) foreign DNA
( c ) selection of recombinants from non – recombinants
( d ) proteins involved in the replication of the plasmid
Ans. d
19. The cutting of DNA at specific locations became possible with the discovery of
( a ) cloning
( b ) restriction endonuclease
( c ) restriction eruyrne
( d ) vectors
Ans. b
20. The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme
( a ) DNA ligase
( b ) RNA polymerase
( c ) DNA polymerase
( d ) All of these
Ans. a
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21. In the year 1963 , the two enzymes responsible for restricting the growth of bacteriophage in E. coli were isolated . One of these added methyl groups to DNA while , the other cut DNA . The latter was called
( a ) restriction endonuclease
( b ) restriction exonuclease
( c ) restriction enume
( d ) plasmid
Ans. a
22. Be toxin is produced by a bacterium called
( a ) Actinomyces
( b ) Streptomyces
( c ) Bacillusthunngiensis
( d ) All of these
Ans. c
23. A method of cellular defences that takes place in all eukaryotic organisms is
( a ) RNA
( b ) downstream processing
( c ) extension
( d ) annealing
Ans. b
24. At present about 30 recombinant therapeutics have been approved for human use the world over How many are presently being marketed in India ?
( a ) 12
( b ) 10
( c ) 30
( d ) 15
Ans. a
25. Insulin consists of two short polypeptide chains , chain A and B , that are linked together by
( a ) hydrogen bond
( b ) disulphide bridges
( C ) DNA Ligase
( d ) DNA polymerase
Ans. b
26. In genetic engineering . DNA fragments are joined through
( a ) ligase
( b ) polymerase
( c ) helicase
( d ) gyrase
Ans. a
27. The most extensively used bacteria in genetic engineering is
( a ) Bacillus
( b ) Clostridium
( c ) Escherichia
( d ) Salmonella
Ans. c
28. PCR is useful in
( a ) DNA synthesis
( b ) DNA amphication
( c ) Protein synthesis
( d ) Amino acid synthes
Ans. b
29. Identify the plasmid
( a ) ECOR
( b ) pBR322
( c ) Alu
( d ) Hind III
Ans. b
30. Extrachromosomal DNA used as vector in gene cloning ist
( a ) transposon
( b ) intron
( c ) exon
( d ) plasmid
Ans. d
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31. DNA segment cleaved by Fco Rl is
( a ) ATTOGA
( b ) GAATTC
( c ) GCTTAA
( d ) CGGCAA
Ans. b
32. Identify the vector suitable for doning long DNA fragments
( a ) phage vector
( b ) bacterial plannid
( c ) yeast plaid
( d ) cosmic
Ans. d
33. Nucleic acid is fragmented by enzyme
( a ) ligases
( b ) proteases
( c ) nucleasen
( d ) polymerases
Ans. c
34. The technique of insertion of a desired gene into DNA of plasmid vector is
( a ) gene splicing
( b ) gene dressing
( c ) gone cloning
( d ) gene drafting
Ans. c
35. Bacterial plasmid contains
( a ) RNA
( b ) RNA + protein
( c ) ONA
( d ) photosynthetic structures
Ans. c
36. Which is the first enzyme used in the production of cDNA
( a ) Restriction onyme
( b ) Reverse transcriptase
( c ) Ligase
( d ) DNA polymerase
Ans. b
37. Which is the first enzyme used in the production of DNA fragments for DNA fingerprinting
( a ) Restriction enzyme
( b ) Lysosomo
( c ) Lyases
( d ) DNA polymerase
Ans. a
38. Which of the following processes or procedures does not involve any nucleic acid hybridisation ?
( a ) Separation of fragments by gel electrophoresis
( b ) Southern blotting
( c ) Polymerase chain reaction
( d ) DNA sequencing by the Sanger method
Ans. a
39. Tag DNA polymerase enzyme , which is the basis of PCR was a declared molecule of the year 1989. It was discovered by
( a ) Karry Mullis
( b ) EM Southern
( c ) Alick Issacs
( d ) J Lindeman
Ans. a
40. Using recombinant DNA technology , genes from a dort cell can be usinted into a bacterium for UNA replication and protein synthesis . The kinds of cells this can be used as ge … anors in this technology are
( a ) bacteria only
( b ) either yeast or bacteria only
( c ) eukaryotic cells only
( d ) any kind of cell
Ans. d
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41. The genes encoding resistance to antibiotics such as ampicillin , chloramphenicol tetracycline or kanamymic etc. are considered useful selectable markers for
( a ) E coll
( b ) Bacillus thuringiensis
( c ) Meloidegyre incognita
( d ) All of the above
Ans. a
42. The procedure , in which DNA fragments are separated by gel electrophoresis and then transferred on to a filter for radioactive probing is known as
( a ) gene mapping
( b ) gene cloning
( c ) polymerase chain reaction
( d ) Southern blotting technique
Ans. d
43. Which of the following restriction enzymes produce blunt ends ?
( a ) Sma I
( b ) Hae III
( c ) Alu I
( d ) All of these
Ans. d
44. Plasmids that carry genes to provide resistance to antibiotics are called
( a ) R plasmids
( b ) C plasmids
( c ) A plasmids
( d ) Ti plasmids
Ans. a
45. The name molecular scissors is given to
( a ) enzymes
( b ) restriction endonucleases
( c ) vectors
( d ) restriction DNAse
Ans. b
46. The breakage of DNA fragment and instting it into another DNA molecule , this technique is related to
( a ) gene splicing
( b ) gene cloning
( c ) gene typing
( d ) DNA fingerprinting
Ans. b
47. Genetically identical copy of individual cell or gene is known as
( a ) species
( b ) phagemid
( c ) clone
( d ) plasmid
Ans. c
48. Which of the following is a cloning vector ?
( a ) Cosmid
( b ) Phagemid
( c ) Plasmids
( d ) All of these
Ans. d
49. Term which can be used as synonym of genetic engineering in many cases is known as
( a ) gene manipulation
( b ) gene therapy
( c ) Both ( a ) and ( b )
( d ) recombinant DNA technology
Ans. d
50. Restriction enzymes were firstly isolated from
( a ) Haemophilus influenzae
( b ) Ecol
( c ) Pseudomonas putida
( d ) Agrobacterium
Ans. b
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51. DNA of plasmid is
( a ) double – stranded and circular
( b ) single – stranded and circular
( c ) double – stranded and linear
( d ) single – stranded and linear
Ans. a
52. Ori sequence in plasmid refers to sequence
( a ) for antibody resistance
( b ) from which replication will start in plasmid
( c ) for restriction site
( d ) Both ( a ) and ( c )
Ans. b
53. PCR stands for
( a ) Polymerase Cyclic Reaction
( b ) Polymerase Chain Reaction
( c ) Polyethyl Cytosine Reaction
( d ) Polymerisation Chain Reaction
Ans. b
54. Yeast has become important in genetic engineering because it
( a ) has plasmids that can be genetically engineered
( b ) allows the study of eukaryotic gene regulation and expression
( c ) grows readily and rapidly in the laboratory
( d ) All of the above
Ans. d
55. The molecular binder in genetic engineering is
( a ) polymerase
( b ) endonuclease
( c ) ligase
( d ) permease
Ans. c
56. Automated DNA sequences , work on the principle of the method developed by
( a ) Fredrick Sanger
( b ) Francis Crick
( c ) Alec Jeffreys
( d ) Erwin Charga
Ans. a
57. A plant species which has been explained for the production of hirudin is
( a ) Brassica napus
( b ) Zea mays
( c ) Solanum nigrum
( d ) Oryza sativa
Ans. a
58. Restriction enzymes are used to cut
( a ) single – stranded RNA
( b ) double – stranded DNA
( c ) single – stranded DNA
( d ) double – stranded RNA
Ans. b
59. A suitable vector for gene cloning in higher organisms is
( a ) baculovirus
( b ) retrovirus
( c ) Salmonella typhimurium
( d ) Neurospora crassa
Ans. a
60. Which of the following is used in recombinant DNA technique ?
( a ) Cell wall of virus
( b ) Gene which produces capsid of virus
( c ) Virus
( d ) Capsid of virus
Ans. c
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61. The first case of IVF – ET technique success , was reported by
( a ) Banting test
( b ) Patrick Steptoe and Robert Edward
( c ) Robert Steptoe and G Brown
( d ) Baylis and Starling Taylor
Ans. b
62. Probes , used in DNA fingerprinting are initially
( a ) single – stranded RNA
( b ) minisatellite
( c ) 19 base long oligonucleotide
( d ) All of the above
Ans. b
63. The vector for T – DNA is
( a ) Thermus aquaticus
( b ) Salmonella typhimurium
( c ) Agrobacterium tumefaciens
( d ) Escherichia coll
Ans. c
64. Which of the following is not a restriction endonuclease ?
( a ) Eco RI
( b ) Hind IlI
( c ) Pst I
( d ) DNAse l
Ans. d
65. The basis of DNA fingerprinting is
( a ) the double helix
( b ) errors in base sequence
( c ) polymorphism in sequence
( d ) DNA replication
Ans. c
